分子生物学研究中心
我和我爸的十七岁分子生物学研究中心主要任务是利用分子生物学、生物化学等现代技术手段开展微生物领域的基础、应用研究及产业化开发。目前,中心有固定人员9人,其中研究员1人,副研究员4人,助理研究员4人,博士5人,陕西省中青年科技领军人才1人,陕西省百优青年科技新星1人。团队主持国家科技部重点研发计划、国家自然基金、陕西省科技厅、中国科学院、西安市科技局、陕西省科学院等纵向课题40多项,获得法国欧莱雅、自然堂、长庆油田等国内外大型企业横向课题10余项。在国内外学术期刊Redox Biology、Nitric Oxide、Microbial Biotechnology、Frontiers in Pharmacology、Biophysics Reports等发表学术论文60余篇,授权国家发明专利6项(美国发明专利2项)。
研究方向:
1.利用基因工程技术,进行高附加值微生物技术产品开发;
2.进行病原菌抗菌分子机制研究、药物靶点开发及抗菌天然活性化合物的筛选;
3.围绕秦巴山区、极端环境及大健康产业,进行微生物新资源的发现、特色微生物资源的收集及其活性天然产物的开发。
平台条件:
实验室面积约500平米。建有微生物资源分离实验室,拥有厌氧工作站、生物安全柜、全温摇床、低温冰箱等仪器设备,保藏高质量秦巴山区、南北极、环境及益生等微生物菌株5000余株,发现潜在新种近百株;建有微生物基因工程实验室,拥有Bio-Rad凝胶成像仪、梯度PCR仪、基因导入仪、蛋白纯化仪等设备20余台套,保藏微生物定向改造菌株质粒100多种;建有天然产物分离、筛选、鉴定平台,拥有液质联用、分析HPLC、半制备HPLC、制备HPLC、高通量移液等设备30余台套。形成从特色微生物资源分离到微生物功能基因和活性天然产物研究等完善的平台体系。
发表部分论文
[1] Lei Gao , Xinhua Qiao, Lixia Zhang , Yan Wang , Yi Wan* , Chang Chen* .Nitric oxide inhibits alginate biosynthesis in Pseudomonas aeruginosa and increases its sensitivity to tobramycin by downregulating algU gene expression. Nitric Oxide. 2022 Nov 1;128:50-58. doi: 10.1016/j.niox.2022.08.001. Epub 2022 Aug 17.
[2] Yuejuan Zhang, Cheng Chen, Bin Cheng, Lei Gao, Chuan Qin , Lixia Zhang, Xu Zhang, Jun Wang, Yi Wan*.Discovery of Quercetin and Its Analogs as Potent OXA-48 Beta-Lactamase Inhibitors.Front. Pharmacol., 22 June 2022 | https://doi.org/10.3389/fphar.2022.926104
[3] Yan Wang, Mao Sun, Fan Chang, Jun Wang, Yan Wang*, Jie Tang, Kun Zhang,Lei Gao, Xiaochang Xue, Yi Wan*.The essential differences in microbial and chemical components of musk of different qualities secreted by captive male forest musk deer (Moschus berezovskii) [J]. Microbial Biotechnology.2022, doi:10.1111/1751-7915.14002.
[4] WANG J, ZHANG X, GAO L, WANG L, SONG F, ZHANG L, WAN Y*. The synergistic antifungal activity of resveratrol with azoles against Candida albicans[J]. Lett Appl Microbiol, 2021 Feb 6, doi: 10.1111/lam.13458.
[5]Wang Lu, Qiao Xinhua, Gao Lei, Chen Chang*, Wan Yi*. A quantitative method to assess bacterial adhesion using recombinant bioluminescent Pseudomonas aeruginosa. Biophysics Reports. 2021, 7(1): 55-70.
[6] HAN J*, YE N, ZHANG X, YU Z, WANG J, ZHANG Y, WAN Y*. Ligand-controlled self-assembly of Ag(I) complexes with cyano-containing ligands and their tunable antimicrobial activities[J]. Inorg Chim Acta, 2020, 512.
[7] WANG S, ZHANG Y, GAO D, ZI J, WANG W, ZHANG N, WAN Y*, WANG L*. Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography[J]. Biotechnology and Bioprocess Engineering, 2019, 24(4): 658-665.
[8] ZHANG K, ZHANG Y, ZI J, XUE X*, WAN Y*. Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues[J]. Biomed Res Int, 2017, 2017: 4817376.
[9] WANG Y, NIE M Q*, WAN Y*, TIAN X T, NIE H Y, ZI J, MA X. Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3[J]. Ann Microbiol, 2017, 67(7): 459-468.
[10] ZHANG X, ZHANG S, WANG J, ZI J, WANG J, CHEN S*, WAN Y*. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System[J]. Biomed Res Int, 2016, 2016: 9469356.
[11] YANG F#, WAN Y#, LIU J, YANG X, WANG H, TAO K, HAN J, SHI J*, HU D*. Expression and purification of rhIL-10-RGD from Escherichia coli as a potential wound healing agent[J]. J Microbiol Methods, 2016, 127: 62-67.
[12] GAO L, ZHANG Y, WANG Y, QIAO X, ZI J, CHEN C*, WAN Y*. Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa[J]. Redox Biol, 2016, 8: 252-258.
[13] ZHANG Y, ZHANG K, WAN Y*, ZI J, WANG Y, WANG J, WANG L*, XUE X. A pH-induced, intein-mediated expression and purification of recombinant human epidermal growth factor in Escherichia coli[J]. Biotechnol Prog, 2015, 31(3): 758-764.
[14] 万一*,訾静,张琨,张志敏,张月娟,王琰,王军. 金黄色葡萄球菌蛋白A(SpA)Z结构域串联体的克隆、表达和筛选[J]. 生物工程学报, 2012, 28(12): 1500-1510.
授权发明专利
[1] Yi Wan, Chang Chen, Lei Gao, Yuying Zhang, Xinhua Qiao, Yan Wang. Method, Target and Application for NO Accumulation to Decrease Pseudomonas Aeruginosa Invasiveness. US10537593,美国发明专利,专利出版公告日:2020年1月21日。
[2] Yi Wan, Chang Chen, Lei Gao, Yuying Zhang, Xinhua Qiao, Yan Wang, Method, Target and Application for NO Accumulation to Decrease Pseudomonas Aeruginosa Invasiveness[P]. US11058716 B2, 美国发明专利,专利出版公告日:2021年7月13日。
[3]万一,陈畅,高磊,张玉英,乔新华,王琰,NO累积降低铜绿假单胞菌侵袭力的方法、靶点及应用,中国,ZL201510697439.9
[4]万一,张琨,张月娟,王琰,一种提高重组人Cu,Zn-SOD活性蛋白产量的方法,2009.3.27,中国,ZL200910021725.8
[5]万一,张月娟,张琨,王琰,訾静,张志敏,王军,一种重组人表皮生长因子的新型制备方法,2012.6.5,中国,ZL201210181522.7
[6]万一,韩丽萍,沈卫荣,张月娟,孙晓宇,张琨,李玥,一种基于抗菌的双功能融合蛋白及其制备方法和用途,2009.10.26,中国,ZL200910209945.3
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